This tutorial demonstrates how to properly prepare, freeze, and store cells to maintain viability and reproducibility for tissue culture experiments.
Whether you are new to cell culture or refreshing your knowledge, this video provides guidance on best practices for freezing cells and handling cell culture samples.
Chapters:
00:00 Introduction to Freezing Cells
00:43 Preparing Conditioned Media and Washing Cells
01:08 Add TrypLE to Dissociate Culture and Detach Cells
01:33 Collecting and Centrifuging Cells
01:40 Add Medium to Cells
02:04 Take a Sample for Cell Counting
02:28 Use the Countess Automated Cell Counter
02:54 Remove Tubes from Centrifuge
03:03 Remove and Discard the Medium
03:13 Resuspend the Pellet for Optimal Freezing Density
03:27 Transfer Cell Solution into Cryovials
03:57 Cells Frozen and Ready for Liquid Nitrogen Tank
04:15 Cryovial Transfer Precautions
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